Multilineage Potential Evaluation Protocol

To assess multilineage potential, cells were incubated under specific conditions to induce differentiation into osteocytes, chondrocytes, and adipocytes. After differentiation, the multilineage potential was evaluated as previously described [21 (link), 22 (link)]. Briefly, osteocyte formation was assessed by measuring the level of ALP staining (Sigma-Aldrich, St. Louis, MO, USA); chondrocyte formation was determined by safranin O staining (Sigma); adipocyte formation was assessed based on the staining of accumulated lipid vacuoles with oil red O (Sigma).

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Kim M., Bae Y.K., Um S., Kwon J.H., Kim G.H., Choi S.J., Oh W, & Jin H.J. (2020). A Small-Sized Population of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Shows High Stemness Properties and Therapeutic Benefit. Stem Cells International, 2020, 5924983.

Publication 2020

ALP staining safranin O oil red O

Adipocyte Cells Chondrocyte Lipid Oil red o Osteocyte Safranin o Vacuoles

Corresponding Organization : Medipost (South Korea)

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Variable analysis

independent variables

Specific conditions to induce differentiation into osteocytes, chondrocytes, and adipocytes

dependent variables

Level of ALP staining (to assess osteocyte formation)
Safranin O staining (to determine chondrocyte formation)
Staining of accumulated lipid vacuoles with oil red O (to assess adipocyte formation)

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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