CDH (EC 1.1.99.18) from Phanerochaete
chrysosporium was heterologously expressed in Trichoderma reesei and purified as described in a
previous study.20 (link) The RZ value (A420/A280) was 0.62
and indicated homogeneous enzyme preparation, exhibiting a specific
activity of 17.5 U mg–1 determined with substrate
cellobiose and 2,6-dichloroindophenol as the electron acceptor at
pH 5.0. Cellulase from T. reesei (Cellulase,
9012-54-8, 0.7 U mg–1), β-glucosidase from Aspergillus niger (9033-06-1, 0.75 U mg–1), Cellic CTec2 (Cellulase, enzyme blend, ∼1.15 g/mL), and
GOx (9001-37-0) from A. niger with
a specific activity of 100–250 U mg–1 were
all purchased from Sigma-Aldrich.
chrysosporium was heterologously expressed in Trichoderma reesei and purified as described in a
previous study.20 (link) The RZ value (A420/A280) was 0.62
and indicated homogeneous enzyme preparation, exhibiting a specific
activity of 17.5 U mg–1 determined with substrate
cellobiose and 2,6-dichloroindophenol as the electron acceptor at
pH 5.0. Cellulase from T. reesei (Cellulase,
9012-54-8, 0.7 U mg–1), β-glucosidase from Aspergillus niger (9033-06-1, 0.75 U mg–1), Cellic CTec2 (Cellulase, enzyme blend, ∼1.15 g/mL), and
GOx (9001-37-0) from A. niger with
a specific activity of 100–250 U mg–1 were
all purchased from Sigma-Aldrich.
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