Liver Lipid Profiling Protocol

Lipids were extracted from liver tissue using chloroform:methanol mixture (2:1 v/v) (40 (link)). enzymatic assay kits were used to measure hepatic and plasma concentrations of triglyceride (Wako Diagnostics). Plasma AST and ALT activities were measured by an enzymatic assay kit (Thermo Fisher Scientific). Plasma insulin and Fgf21 concentrations were determined using commercially available mouse ELISA kits from Crystal Chem and R&D Systems, respectively, according to the manufacturer's specification. Protein concentrations were determined using a bicinchoninic acid assay (Thermo Fisher Scientific).

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Krumm C.S., Xu X., Bare C.J., Holman C.D., Kersten S., Dow L.E., Lee A.H, & Cohen D.E. (2021). Inducible hepatic expression of CREBH mitigates diet-induced obesity, insulin resistance, and hepatic steatosis in mice. The Journal of Biological Chemistry, 297(1), 100815.

Publication 2021

Enzymatic assay kits enzymatic assay kit mouse ELISA kits bicinchoninic acid assay

Assay Bicinchoninic acid Chloroform Diagnostics Elisa Enzymatic assay Fgf21 Insulin Lipids Methanol Mouse Plasma Protein Triglyceride

Corresponding Organization : Cornell University

Other organizations : Wageningen University & Research

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Variable analysis

independent variables

Lipid extraction method (chloroform:methanol mixture (2:1 v/v))

dependent variables

Hepatic triglyceride concentration
Plasma triglyceride concentration
Plasma AST activity
Plasma ALT activity
Plasma insulin concentration
Plasma Fgf21 concentration
Protein concentration

control variables

Liver tissue

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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