Inflammation Modulation by Omega-3 Lipids

Purified eicosapentaenoic acid and docosahexaenoic acid, palmitic acid, and oleic acid were obtained from Sigma Aldrich. Epanova® (omega-3-carboxylic acids [OM-3 CA]) was supplied by AstraZeneca. Over-the-counter omega-3-triglyceride supplements (Nordic Naturals) were purchased from Amazon. Safflower oil was purchased from a commercial grocer. Colchicine, indomethacin, and Ac-YVAD-cmk (YVAD) were purchased from Sigma Chemical. MSU, cholesterol, and calcium pyrophosphate crystals were made according to established protocols^{31 (link),32 (link)}. Crystals were tested and found to be free of endotoxins using PYROGENT™–5000 Kinetic Turbidimetric Limulus Amebocyte Lysate assays (Lonza). Cell-culture supernatant and pouch exudates were analysed for IL-1β content with electrochemiluminescence enzyme-linked immunosorbent assay (ELISA) plates (Mesoscale). Prostaglandin E₂ (PGE₂) content was analysed using a competitive enzyme immunoassay (R&D Systems).

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Iverson C., Bacong A., Liu S., Baumgartner S., Lundström T., Oscarsson J, & Miner J.N. (2018). Omega-3-carboxylic acids provide efficacious anti-inflammatory activity in models of crystal-mediated inflammation. Scientific Reports, 8, 1217.

Publication 2018

docosahexaenoic acid palmitic acid oleic acid Colchicine indomethacin Ac-YVAD-cmk (YVAD) competitive enzyme immunoassay

Ac yvad cmk Amebocyte lysate Assays Calcium pyrophosphate Cell culture Cholesterol Colchicine Docosahexaenoic acid Eicosapentaenoic acid Endotoxins Enzyme immunoassay Enzyme linked immunosorbent assay Exudates Il 1β Indomethacin Kinetic Limulus Oleic acid Omega 3 Omega 3 carboxylic acids Over the counter Palmitic acid Prostaglandin e2 Safflower oil Supplements Triglyceride Turbidimetric

Corresponding Organization :

Other organizations : AstraZeneca (Sweden)

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Variable analysis

independent variables

Purified eicosapentaenoic acid and docosahexaenoic acid
Palmitic acid
Oleic acid
Epanova® (omega-3-carboxylic acids [OM-3 CA])
Over-the-counter omega-3-triglyceride supplements (Nordic Naturals)
Safflower oil
Colchicine
Indomethacin
Ac-YVAD-cmk (YVAD)
Cholesterol
Calcium pyrophosphate crystals

dependent variables

IL-1β content in cell-culture supernatant and pouch exudates
Prostaglandin E2 (PGE2) content

control variables

Crystals were tested and found to be free of endotoxins

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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