Comprehensive Antibody Panel for HBV

Primary antibodies specific for HBV proteins included anti-HBV core protein mouse monoclonal antibody (MAb) 8C9-11 (72 (link)), anti-HBV core protein mouse MAb sc-23945 (Santa Cruz), both recognizing linear epitopes within the HBV core protein, and HBV capsid/capsid-intermediate-specific rabbit polyclonal antibody (PAb) B0586 (Dako). Primary antibodies used for epitope tags and cellular targets included anti-HA rat MAb 3F10 (Core Facility for Monoclonal Antibodies, Helmholtz Zentrum München), mouse MAb against the His₆ tag (Clontech), anti-PML protein mouse MAb sc-966 (Santa Cruz), polyclonal rabbit anti-PML protein PAb NB100-59787 (Novus Biologicals), polyclonal rabbit anti-PML protein ab72137 (Abcam), monoclonal mouse anti-SUMO2/3 ab81371 (Abcam), and anti-beta-actin mouse MAb AC-15 (Sigma-Aldrich). Secondary antibodies conjugated to Alexa Fluor 488 were purchased from Thermo Scientific, and secondary antibodies coupled to horseradish peroxidase or Alexa Fluor 647 were anti-rabbit IgG, anti-mouse IgG, anti-mouse light chain IgG, and anti-rat IgG (all from Dianova).

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Hofmann S., Plank V., Groitl P., Skvorc N., Hofmann K., Luther J., Ko C., Zimmerman P., Bruss V., Stadler D., Carpentier A., Rezk S., Nassal M., Protzer U, & Schreiner S. (2023). SUMO Modification of Hepatitis B Virus Core Mediates Nuclear Entry, Promyelocytic Leukemia Nuclear Body Association, and Efficient Formation of Covalently Closed Circular DNA. Microbiology Spectrum, 11(3), e00446-23.

Publication 2023

sc-23945 sc-966 NB100-59787 ab72137 anti-SUMO2/3 ab81371 AC-15 anti-rabbit IgG anti-mouse IgG

Alexa fluor 488 Alexa fluor 647 Anti antibody Anti igg Antibodies Antibody Beta actin Biologicals Capsid Cellular Epitope His6 tag Horseradish peroxidase Light Monoclonal antibody Mouse Novus Protein Protein monoclonal Rabbit

Corresponding Organization : Helmholtz Zentrum München

Other organizations : University Medical Center Freiburg, Center for Experimental and Clinical Infection Research, Alexandria University, German Center for Infection Research

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Variable analysis

independent variables

Primary antibodies specific for HBV proteins, including anti-HBV core protein mouse monoclonal antibody (MAb) 8C9-11, anti-HBV core protein mouse MAb sc-23945, and HBV capsid/capsid-intermediate-specific rabbit polyclonal antibody (PAb) B0586

dependent variables

Not explicitly mentioned

control variables

Secondary antibodies conjugated to Alexa Fluor 488, horseradish peroxidase, or Alexa Fluor 647

positive controls

Anti-HA rat MAb 3F10, mouse MAb against the His6 tag, anti-PML protein mouse MAb sc-966, polyclonal rabbit anti-PML protein PAb NB100-59787 and ab72137, monoclonal mouse anti-SUMO2/3 ab81371, and anti-beta-actin mouse MAb AC-15

negative controls

Not specified

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