Immunostaining Protocol for IgG4 Quantification

Blinded to the IgG4 quantification, two authors (C.K. and S.G.A.) analyzed one haematoxylin and eosin (H&E) slide from each case sectioned from the same paraffin-embedded tissue cassette as were the immunostained slides. The parameters assessed were: a dense lymphoplasmacytic infiltrate, storiform fibrosis, obliterative phlebitis, epithelioid histiocytes, granulomas, giant cells, and neutrophils. Immunohistochemistry staining was performed as described previously (24 (link)). Specific to this study, slides were incubated for 1.5 hours at room temperature with an antibody cocktail containing rabbit anti-IgG (Cell Marque, Cat# 269A-15) diluted 1:5400 and mouse anti-IgG4 (Cell Marque, Cat# 367M-15) diluted 1:900 and detected with Mach 2 Double Stain 2, (Biocare Medical) for 10 minutes at room temperature.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Allen S.G., Soliman A.S., Toy K., Omar O.S., Youssef T., Karkouri M., Ayad E., Abdel‐Aziz A., Hablas A., Tahri A., Oltean H.N., Kleer C.G, & Merajver S.D. (2016). Chronic Mastitis in Egypt and Morocco: Differentiating between Idiopathic Granulomatous Mastitis and IgG4‐Related Disease. The Breast Journal, 22(5), 501-509.

Publication 2016

rabbit anti-IgG mouse anti-IgG4 Mach 2 Double Stain 2

Anti igg Antibody cocktail Cell Embedded paraffin Eosin Fibrosis Giant cells Granulomas Haematoxylin Histiocytes Igg4 Mach 2 Mouse Neutrophils Phlebitis Rabbit Stain Tissue

Corresponding Organization : University of Michigan–Ann Arbor

Other organizations : University of Nebraska at Omaha, Cairo University, Mansoura University, University of Hassan II Casablanca, Centre Hospitalier Universitaire Mohammed VI

Top 5 similar protocols

Variable analysis

Dependent Variables not detected.
Unable to provide accurate variables.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!