Rhodamine-labeled ApoEVs were obtained by modifying rhodamine-labeled (DSS)6 onto the surface of ApoEVs, PKH26-labeled ApoEVs were obtained by pre-labeling with the PKH26 Red Fluorescent Cell Linker Kit (Sigma, MINI26) according to the manufacturer’s instructions.
The BMSCs (P2) were plated on confocal dishes and maintained in a cell incubator. When the cells reached 50–60% confluence, ApoEVs labeled with Rhodamine or PKH26 red fluorescence were added to the BMSCs at a concentration of 10 μg/mL for 12 h. After fixation with 4% paraformaldehyde, cells were stained with fluorescein isothiocyanate-phalloidin (FITC-Phalloidin) (Yeasen, 40735ES75) and cell nuclei staining solution (Hoechst 33342) (Sigma, 14533). Fluorescence was observed using CLSM.
The BMSCs (P2) were plated on confocal dishes and maintained in a cell incubator. When the cells reached 50–60% confluence, ApoEVs labeled with Rhodamine or PKH26 red fluorescence were added to the BMSCs at a concentration of 10 μg/mL for 12 h. After fixation with 4% paraformaldehyde, cells were stained with fluorescein isothiocyanate-phalloidin (FITC-Phalloidin) (Yeasen, 40735ES75) and cell nuclei staining solution (Hoechst 33342) (Sigma, 14533). Fluorescence was observed using CLSM.
