Substrate Surface Free Energy Determination

Surface free energies of the substrate/air interface and WCA were determined by the sessile drop method using the Drop Shape Analyzer—DSA25 (Krüss, Hamburg, Germany) (Staehlke et al., 2018 (link)). One μl drops of distilled water and diiodomethane (Sigma-Aldrich Chemie, Taufkirchen, Germany) were deposited on the sample surface (n = 3 at 3 drops each). WCA values were calculated with the supplied software (ADVANCE, V.1.7.2.1, Krüss, Hamburg, Germany) via the Young’s equation and the SFE according to Owens, Wendt, Rabel und Kaelble (OWRK).

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Gruening M., Neuber S., Nestler P., Lehnfeld J., Dubs M., Fricke K., Schnabelrauch M., Helm C.A., Müller R., Staehlke S, & Nebe J.B. (2020). Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges. Frontiers in Bioengineering and Biotechnology, 8, 1016.

Publication 2020

Drop Shape Analyzer—DSA25 diiodomethane

Diiodomethane

Corresponding Organization : University of Rostock

Other organizations : Universität Greifswald, University of Regensburg, Innovent, Leibniz Institute for Plasma Science and Technology

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Surface free energies of the substrate/air interface
Water contact angle (WCA)

control variables

Distilled water and diiodomethane as the liquids used for contact angle measurements
Sample surface for depositing the 1 μl drops (n = 3 at 3 drops each)

controls

No positive or negative controls were explicitly mentioned in the input text.

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