Serum Fatty Acid Profiling by GC-MS/MS

Gas chromatography-tandem mass spectrometry (GC-MS/MS) was used to analyze 37 fatty acids in serum, conducted in accordance with well-established protocols, which have been previously described in detail (Stupin et al., 2020 (link)). In brief, fatty acids were identified using the GC-MS/MS system by Thermo Fisher GC Trace 1300 coupled with a TSQ 9000 Triple Quadrupole (Thermo Fisher Scientific, Waltham, MA, USA), and the fatty acids profile was analyzed at the BIOCentre’s Bioanalytical Laboratory, BIOCentre－Incubation Center for Biosciences, Zagreb, Croatia.

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Kolar L., Stupin M., Stupin A., Šušnjara P., Mihaljević Z., Matić A., Jukić I., Kolobarić N, & Drenjančević I. (2021). Does the Endothelium of Competitive Athletes Benefit from Consumption of n-3 Polyunsaturated Fatty Acid-Enriched Hen Eggs?. Preventive Nutrition and Food Science, 26(4), 388-399.

Publication 2021

GC Trace 1300

Fatty acids Gc ms Ms ms Serum

Corresponding Organization :

Other organizations : University of Osijek

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Fatty acids profile in serum

control variables

Well-established protocols for gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis of 37 fatty acids in serum, which have been previously described in detail (Stupin et al., 2020)

controls

No positive or negative controls were explicitly mentioned in the given information.

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