Mice with Id3 conditional knockout(35 (link)) and LckCre transgenic(36 (link)) alleles have been described previously. Egr1−/−, Egr2f/f and Egr3−/− mice were as previously reported(37 (link)). All the strains are on B6 background. Animals were bred and maintained in the SPF facility managed by Duke University Division of Laboratory Animal Research. Animal procedures were approved by the Duke University Institutional Animal Care and Use Committee.
The antibodies used were as follows: FITC anti-mouse TCRδ (GL3), PE/Cy7 anti-mouse TCRδ (GL3), PE/Cy5 anti-mouse NK1.1 (PK136), PE/Cy7 anti-mouse/human CD44 (IM7), APC anti-mouse CD24 (M1/69), PE-Cy7 anti-mouse IFNγ (XMG1.2), and Pacific Blue™ anti-mouse IL-17 (TC11-18H10.1) were purchased from Biolegend; PE anti-mouse Vδ6.3/2 (8F4H7B7) APC anti-mouse PLZF (9E12) and APC BrdU Flow Kit were purchased from BD Biosciences. Rabbit anti-mouse Egr2 (EPR4004) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488, ab150077) were purchased from abcam.
The antibodies used were as follows: FITC anti-mouse TCRδ (GL3), PE/Cy7 anti-mouse TCRδ (GL3), PE/Cy5 anti-mouse NK1.1 (PK136), PE/Cy7 anti-mouse/human CD44 (IM7), APC anti-mouse CD24 (M1/69), PE-Cy7 anti-mouse IFNγ (XMG1.2), and Pacific Blue™ anti-mouse IL-17 (TC11-18H10.1) were purchased from Biolegend; PE anti-mouse Vδ6.3/2 (8F4H7B7) APC anti-mouse PLZF (9E12) and APC BrdU Flow Kit were purchased from BD Biosciences. Rabbit anti-mouse Egr2 (EPR4004) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488, ab150077) were purchased from abcam.
