Multiplex Mycotoxin Detection Assay

Methanol and acetonitrile (HPLC grade) were purchased from Merck Drugs and Biotechnology (New York, NY, USA). AFB₁, AFB₂, AFG₁, AFG₂, OTA, zearalenone (ZEN), trichothecenes (T₂), deoxynivalenol (DON), fumonisins (FB) standard solutions, goat anti-mouse IgG, bovine serum albumin (BSA), and ovalbumin (OVA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium borate decahydrate, potassium carbonate, sucrose, Tween-20, acetic acid, sodium azide, sodium chloride, sodium hydrogen phosphate, potassium dihydrogen phosphate, potassium chloride, hydrochloric acid, and sodium bicarbonate were purchased from Damao Chemical Reagent Factory (Tianjin, China). Colloidal gold (particle size ~60 mm) and AFB₁/OTA monoclonal antibody were purchased from Clover Technology Group Inc. (Beijing, China).
The following reagents were used: PBS; boric acid buffer solution (0.2 mol/L); resuspension solution, gold label pad treatment solution, sample pad treatment solution, sample buffer [23 (link)].