Pemetrexed was purchased from LC Laboratories (Woburn, MA). FTY720 was purchased from Cayman Chemical Inc., (Ann Arbor MI). Sorafenib tosylate and all ERBB receptor and kinase inhibitors were purchased from Selleckchem (Houston, TX). Trypsin-EDTA, DMEM, RPMI, penicillin-streptomycin were purchased from GIBCOBRL (GIBCOBRL Life Technologies, Grand Island, NY). Cells were purchased from the ATCC and were not further validated beyond that claimed by ATCC. Cells were re-purchased every ~6 months. The plasmid to express GRP78/BiP/HSPA5 was kindly provided to the Dent laboratory by Dr. A.S. Lee (University of Southern California, Los Angeles, CA); all other plasmids were purchased from Addgene. Commercially available validated short hairpin RNA molecules to knock down RNA / protein levels were from Qiagen (Valencia, CA) or were supplied by collaborators. Rabbit antiserum for mouse PERK phosphorylated at T799 was developed as described [44 (link)]. Reagents and performance of experimental procedures were described in refs: [12 (link)/13 (link)/17 (link)/18 (link)/21 (link)–24 (link)/33 (link)/40 (link)].
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