amygdalin from apricot kernels (Sigma-Aldrich, Taufkirchen, Germany) was freshly dissolved in tumor cell culture medium and then added to tumor cells at a concentration of 10 mg/ml for either 24 h or for 2 weeks [10] (link) to evaluate acute versus chronic treatment. Controls received tumor cell culture medium alone. In all experiments, treated tumor cell cultures were compared to the non-treated ones. To exclude toxic effects of amygdalin, cell viability was determined by trypan blue (Gibco/Invitrogen).
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