Total RNA was isolated as described (6 (link)) at 14 or 18 h postinfection (hpi) as indicated. Templates for Northern probes were amplified by PCR from viral or mouse genomic DNA (using PCR primers listed in Table S2 in the supplemental material), prepared as described in reference 6 (link) for probes 2 and 7 (formerly EGR 26 probes 1 and 4 [6 (link)], respectively), or obtained from a commercial vendor for actin (Life Technologies). Northern blotting using Ambion’s NorthernMax kit (Life Technologies, Grand Island, NY) and generation of single-stranded P32-labeled RNA probes using the Maxiscript Sp6/T7 kit (Life Technologies) were performed as described previously (6 (link)). Probe 12 was generated using the mirVANA miRNA probe construction kit (Life Technologies) according to the manufacturer’s instructions. Five micrograms of total RNA was used for all Northern blot analyses unless otherwise stated. Membranes were scanned using a Storm 840 Phosphorimager and quantitated using ImageQuant TL (GE Healthcare Biosciences, Pittsburgh, PA). For quantitation of each sample, the indicated transcript signal was normalized to the signal from the actin loading control.