All human data was collected in accordance with the declaration of Helsinki and Ethical approval was granted by the Landesärztekammer Rheinland-Pfalz (Mainz, Germany; permit number: 837.190.12 (8291-F)). Written consent was received from all included individuals. Human data are previously unpublished results of the FLuG-Risiko study.7 (link) Serum of each individual was sampled after nights without or with aircraft noise exposure for 6 h. The aircraft noise consisted of 60 repetitive noise events, which had been recorded near Düsseldorf airport, interrupted by random silent periods, yielding a mean sound pressure level of 47 dB(A) (compared to 39 dB(A) in the silent nights). The detailed protocol and health status of included individuals was published previously.7 (link)
All animals were treated in accordance with the Guide for the Care and Use of Laboratory Animals as adopted by the U.S. National Institutes of Health and approval was granted by the Ethical Committee of the University Medical Center Mainz and the Landesuntersuchungsamt Rheinland-Pfalz (Koblenz, Germany; permit number: 23 177-07/G 15-1-094). After the indicated duration and protocol of noise exposure, animals were killed under isoflurane anaesthesia by transection of the diaphragm and removal of the heart and thoracic aorta. Glucose levels were assessed in whole blood using the ACCU-CHEK Sensor system from Roche Diagnostics GmbH (Mannheim, Germany).
For detailed description of all methods, see Supplementary material online.