CLP and Anti-TIGIT Therapy in Listeria Model

CLP was performed 60 days after L. monocytogenes infection. Under isoflurane anesthesia, a midline incision was performed, and the cecum was externalized. The cecum was ligated with a 4-0 silk suture and punctured through and through with a 25-gauge needle. All animals received buprenorphine (0.1 mg/kg; McKesson Medical) preoperatively for pain relief. Immediately after surgery, animals received 1 mL sterile saline for fluid resuscitation as well as antibiotics (50 mg/kg ceftriaxone and 35 mg/kg metronidazole, Sigma-Aldrich) every 12 hours for 2 days. Mice were randomized to receive 400 μg anti-TIGIT blocking mAb (clone 1G9, BioXcell) (18 (link)) or isotype control Ab (mouse IgG, clone MOPC-21, BioXcell) via i.p. injection at 12 and 24 hours after CLP. All animals were euthanized with CO₂ asphyxiation at the indicated time points.