Homozygous 3 × Tg mice, harboring PS1(M146V), APP(Swe), and tau(P301L) transgenes were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and bred in our animal facility. These mice exhibit memory deficits associated with amyloid plaques deposition and tangle pathology [105 (link)].
Randomly chosen, male 4-month-old transgenic mice and age-matched male C57Bl/6 mice (wild-type) were divided into four groups (14 mice in each group) and housed in individually ventilated cages (Lab Products Inc., Seaford, DE, USA), with five mice per cage. The animals were provided with water and food ad libitum. The control animals received regular water. The experimental mice received water with dissolved (250 mg/L) norvaline (Sigma, St. Louis, MO, USA) for ten weeks in accordance with the previously published protocol [16 (link)]. The experiment lasted 2.5 months. The experiments were performed according to the “Guide for the Care and Use of Laboratory Animals” [106 (link)] and the experimental protocol (No 55-07-2018) was approved by Bar-Ilan University animal care and use committee.
Randomly chosen, male 4-month-old transgenic mice and age-matched male C57Bl/6 mice (wild-type) were divided into four groups (14 mice in each group) and housed in individually ventilated cages (Lab Products Inc., Seaford, DE, USA), with five mice per cage. The animals were provided with water and food ad libitum. The control animals received regular water. The experimental mice received water with dissolved (250 mg/L) norvaline (Sigma, St. Louis, MO, USA) for ten weeks in accordance with the previously published protocol [16 (link)]. The experiment lasted 2.5 months. The experiments were performed according to the “Guide for the Care and Use of Laboratory Animals” [106 (link)] and the experimental protocol (No 55-07-2018) was approved by Bar-Ilan University animal care and use committee.
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