MDCK-SIAT2,6-UF Cells for Influenza Virus Isolation

MDCK-SIAT2,6-UF cells [10 , 11 (link)], which overexpress influenza virus α2,6-linked sialic acid receptors, were used for the isolation of influenza viruses. The cells were propagated as monolayers at 37 °C and 5% CO2 in Advanced Dulbecco’s Modified Eagle’s Medium (Invitrogen Corp., Carlsbad, CA, USA) supplemented with 2 mM L-Alanyl-L-Glutamine (GlutaMAX, Invitrogen Corp.), antibiotics (50 μg/mL penicillin, 50 μg/mL streptomycin, 100 μg/mL neomycin (Invitrogen Corp.)), and 10% (v/v) low IgG, heat-inactivated gamma-irradiated fetal bovine serum (HyClone, Logan, Utah).

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Rand K.H., Pieretti M., Arcenas R., Beal S.G., Houck H., Boslet E, & Lednicky J.A. (2017). Semi-quantitative Influenza A population averages from a multiplex respiratory viral panel (RVP): potential for reflecting target sequence changes affecting the assay. Virology Journal, 14, 128.

Publication 2017

Advanced Dulbecco’s Modified Eagle’s Medium penicillin streptomycin neomycin

Alanyl l glutamine Antibiotics Cells Eagle Fetal bovine serum Gamma Influenza viruses Isolation Neomycin Penicillin Sialic acid receptors Streptomycin

Corresponding Organization : University of Florida

Other organizations : BayCare Health System, Materials Systems (United States), National Human Genome Research Institute, National Institutes of Health, University of Florida Health

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Variable analysis

independent variables

MDCK-SIAT2,6-UF cells which overexpress influenza virus α2,6-linked sialic acid receptors

dependent variables

Isolation of influenza viruses

control variables

Cells were propagated as monolayers at 37 °C and 5% CO2
Advanced Dulbecco's Modified Eagle's Medium (Invitrogen Corp., Carlsbad, CA, USA) supplemented with 2 mM L-Alanyl-L-Glutamine (GlutaMAX, Invitrogen Corp.), antibiotics (50 μg/mL penicillin, 50 μg/mL streptomycin, 100 μg/mL neomycin (Invitrogen Corp.)), and 10% (v/v) low IgG, heat-inactivated gamma-irradiated fetal bovine serum (HyClone, Logan, Utah)

controls

No positive or negative controls were explicitly mentioned.

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