Fisetin was purchased from Sigma–Aldrich (≥98% purity by HPLC; Sigma-Aldrich, St. Louis, MO, USA). Cell viability was detected using the cell counting kit-8 assay kit (CCK-8, Sigma) as described previously [18 (link)]. Briefly, BEAS-2B cells were seeded in a 96-well culture plate at a density of 1 × 103 cells/well. The cells were treated with 0–100 μM Fisetin for 24 h. Next, CCK-8 detection solution was added to the culture plate and incubated at 37 °C for 4 h. Cell viability was detected using a multimode reader (Thermo, Waltham, MA, USA) based on the absorbance at 450 nm.
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