Quantifying Trichoderma reesei Biomass

The biomass of T. reesei grown in TMM + 2% cellulose was indirectly determined by the amount of intracellular protein [46 (link), 47 (link)]. In brief, the mycelia of T. reesei were suspended in 1 M NaOH and incubated for 2 h with frequent vortex. Then the protein concentration of the supernatant of the suspension was determined by the Modified BCA Protein Assay Kit (Sangon Biotech, Shanghai, China). The biomass dry weight was calculated assuming an average content of 0.32 g intracellular protein per g of dry cell mass.

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Pang A.P., Luo Y., Hu X., Zhang F., Wang H., Gao Y., Durrani S., Li C., Shi X., Wu F.G., Li B.Z., Lu Z, & Lin F. (2022). Transmembrane transport process and endoplasmic reticulum function facilitate the role of gene cel1b in cellulase production of Trichoderma reesei. Microbial Cell Factories, 21, 90.

Publication 2022

Modified BCA Protein Assay Kit

Assay Cellulose G cell G protein Intracellular Mycelia Protein Protein g

Corresponding Organization :

Other organizations : Southeast University, Nanjing Forestry University, Tianjin University

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Variable analysis

independent variables

Growth medium (TMM + 2% cellulose)

dependent variables

Intracellular protein concentration
Biomass dry weight

control variables

Incubation time (2 hours)
NaOH concentration (1 M)
Vortexing frequency

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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