Tumor Growth Inhibition via Honokiol and Leptin

MDA-MB-231 cells xenografts were generated; as previously described [28 (link)], grouped in four experimental groups. Mice were treated with intraperitoneal (IP) injections of 1) control (saline and Intralipid); 2) HNK, at 3 mg/mouse/day in 20% Intralipid (Baxter Healthcare, Deerfield, IL), three times per week; 3) recombinant leptin (dosage of 5 mg/kg), 5 days a week; 4) leptin and HNK for 4 weeks. The dose and route of HNK and leptin administration was selected from our previous studies documenting in vivo efficacy of honokiol and leptin [17 (link), 28 (link)]. Tumors were regularly measured; collected after 4 weeks of treatment, weighed, and subjected to further analysis. These tumors were utilized for examining the expression of Vimentin, Fibronectin, Zeb1, Zeb2, Slug and actin. All animal studies were in accordance with the guidelines of Johns Hopkins University IACUC.

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Avtanski D.B., Nagalingam A., Bonner M.Y., Arbiser J.L., Saxena N.K, & Sharma D. (2015). Honokiol activates LKB1-miR-34a axis and antagonizes the oncogenic actions of leptin in breast cancer. Oncotarget, 6(30), 29947-29962.

Publication 2015

Intralipid

Actin Animal Fibronectin Honokiol Iacuc Intralipid Intraperitoneal injections Leptin Mda mb 231 cells Mouse Saline Slug Tumors Vimentin Xenografts

Corresponding Organization :

Other organizations : Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center, Emory University, United States Department of Veterans Affairs, University of Maryland, Baltimore

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Variable analysis

independent variables

Treatment with control (saline and Intralipid)
Treatment with HNK at 3 mg/mouse/day in 20% Intralipid, three times per week
Treatment with recombinant leptin at a dosage of 5 mg/kg, 5 days a week
Treatment with leptin and HNK for 4 weeks

dependent variables

Tumor weight
Expression of Vimentin, Fibronectin, Zeb1, Zeb2, Slug, and actin in the tumors

control variables

MDA-MB-231 cells xenografts in mice
4 weeks of treatment duration

positive controls

None specified

negative controls

Control group treated with saline and Intralipid

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