Quantifying Bacterial Cell Motility
Corresponding Organization :
Other organizations : Lawrence Berkeley National Laboratory, University of Missouri
Variable analysis
- Sulfate (30 mM)
- Lactate (60 mM)
- PBS (1X, control)
- Cell mass that entered the capillary during the assay, as measured by the micro-BCA assay
- Culture OD (approximately 0.4-0.5, mid-log phase)
- Resuspension in PBS
- Capillary dimensions (32 mm length, 1.1 mm inner diameter)
- Incubation time (15 min)
- Negative control: Capillary filled with 1X PBS
Annotations
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