Comprehensive RhoA Signaling Toolkit

Raichu-RhoA (provided by M. Matsuda [27 (link)]), pTriEx-RhoA FLARE.sc Biosensor WT (RRID:Addgene_12150), pTriEx-RhoA FLARE.sc Biosensor Q63L (RRID:Addgene_12151), pTriEx-RhoA FLARE.sc Biosensor T19N (RRID:Addgene_12152), pRK5-myc-RhoA WT (RRID:Addgene_12962), pRK5-myc-RhoA Q63L (RRID:Addgene_12964), pRK5-myc-RhoA T19N (RRID:Addgene_12963), EGFP-p65 (RRID:Addgene_111190), GW1-pHRed (RRID:Addgene_31473), GW1CMV-Perceval (RRID:Addgene_21737), Laconic/pcDNA3.1 (+) (RRID:Addgene_118627), Pyronic /pcDNA3.1 (+) (RRID:Addgene_51308), pcDNA3.1 FLII12Pglu-700uDelta6 (RRID:Addgene_17866), Cyto-ABKAR (RRID:Addgene_61510), pLentiEKAR2G2 (RRID:Addgene_40178), Kras-Src FRET biosensor (RRID:Addgene_78302), pFRET-HSP33 cys (RRID:Addgene_16076), pGP-CMV-GCaMP6F (RRID:Addgene_40755), mCherry-Lifeact-7 (RRID:Addgene_54491), pMitoTimer (RRID:Addgene_52659), pCLBW cox8 EGFP mCherry (RRID:Addgene_78520).

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Socodato R., Rodrigues-Santos A., Tedim-Moreira J., Almeida T.O., Canedo T., Portugal C.C, & Relvas J.B. (2023). RhoA balances microglial reactivity and survival during neuroinflammation. Cell Death & Disease, 14(10), 690.

Publication 2023

pTriEx-RhoA FLARE pRK5-myc-RhoA T19N EGFP-p65 GW1-pHRed pLentiEKAR2G2 pGP-CMV-GCaMP6F mCherry-Lifeact-7 pMitoTimer

Biosensor Fret Kras Rhoa

Corresponding Organization : i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto

Other organizations : Universidade do Porto

Top 5 similar protocols

Variable analysis

independent variables

Raichu-RhoA (provided by M. Matsuda [27 (link)])
PTriEx-RhoA FLARE.sc Biosensor WT (RRID:Addgene_12150)
PTriEx-RhoA FLARE.sc Biosensor Q63L (RRID:Addgene_12151)
PTriEx-RhoA FLARE.sc Biosensor T19N (RRID:Addgene_12152)
PRK5-myc-RhoA WT (RRID:Addgene_12962)
PRK5-myc-RhoA Q63L (RRID:Addgene_12964)
PRK5-myc-RhoA T19N (RRID:Addgene_12963)
EGFP-p65 (RRID:Addgene_111190)
GW1-pHRed (RRID:Addgene_31473)
GW1CMV-Perceval (RRID:Addgene_21737)
Laconic/pcDNA3.1 (+) (RRID:Addgene_118627)
Pyronic /pcDNA3.1 (+) (RRID:Addgene_51308)
PcDNA3.1 FLII12Pglu-700uDelta6 (RRID:Addgene_17866)
Cyto-ABKAR (RRID:Addgene_61510)
PLentiEKAR2G2 (RRID:Addgene_40178)
Kras-Src FRET biosensor (RRID:Addgene_78302)
PFRET-HSP33 cys (RRID:Addgene_16076)
PGP-CMV-GCaMP6F (RRID:Addgene_40755)
MCherry-Lifeact-7 (RRID:Addgene_54491)
PMitoTimer (RRID:Addgene_52659)
PCLBW cox8 EGFP mCherry (RRID:Addgene_78520)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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