Evaluating Mitochondrial Proteins in Cells

Levels of SDHA and COX-1 (MT-CO1) proteins were evaluated with MitoBiogenesis™ In-Cell ELISA Kit (Abcam) on Fluostar plate reader (OMEGA, BMG LABTECH), according to the manufacture’s protocol, after 5 days of exposure to BZ. The results are shown in the graph as the [%] of the untreated control. The total number of cells was determined with Janus green and calculated from a standard curve. The details of the measurement have been described previously [11 (link), 12 (link)].

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Augustyniak J., Lenart J., Gaj P., Kolanowska M., Jazdzewski K., Stepien P.P, & Buzanska L. (2018). Bezafibrate Upregulates Mitochondrial Biogenesis and Influence Neural Differentiation of Human-Induced Pluripotent Stem Cells. Molecular Neurobiology, 56(6), 4346-4363.

Publication 2018

MitoBiogenesis™ In-Cell ELISA Kit Fluostar plate reader

Cell Cox 1 Elisa Janus green Proteins

Corresponding Organization :

Other organizations : Mossakowski Medical Research Institute, Polish Academy of Sciences, Polish Academy of Sciences, University of Warsaw, Medical University of Warsaw

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Variable analysis

independent variables

Exposure to BZ

dependent variables

Levels of SDHA protein
Levels of COX-1 (MT-CO1) protein

control variables

Total number of cells

controls

Untreated control

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