IHC was performed on selected sections, as described [20 (link), 22 (link)]. Primary antibodies were: histone H3-K27M (Millipore/Sigma, Burlington, MA), IDH1-R132H (DIA-H09, Dianova, Hamburg, Germany), p53 (DO-7), vimentin (V9), Ki-67 (30–9) (Roche/Ventana, Tucson, AZ), Olig-2 (387 M-15), GFAP (EP672Y) (Ventana/Cell Marque, Rocklin, CA).
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