FSCV Electrochemical Measurements Setup

A two-electrode setup (a working electrode versus a quasi Ag/AgCl reference electrode) was utilized in a custom flow injection cell for FSCV experiments. A self-constructed potentiostat with a variable gain headstage (50 nA/V, 100 nA/V, 200 nA/V, 500 nA/V, 1 µA/V) was connected to the electrode to carry out measurements. Data were collected using a NI-6363 data acquisition card and HDCV software (Version 4, Department of Chemistry, University of North Carolina, Chapel Hill, NC, USA) [40 (link)]. For all experiments, the flow injection system used a TTL voltage-controlled source to switch a six-way HPLC valve to introduce a bolus of test analyte. A flow rate of 0.75 mL min⁻¹ was used to deliver aCSF buffer by a NE-1000 syringe pump (New Era Pump Systems, Inc., Farmingdale, NY, USA).