Apatinib Modulates PKM2 Expression in Thyroid Cancer Cells

SW579 and TPC-1 cells were first disposed of 20 ng/ml, 50 ng/ml, 100 ng/ml Apatinib (Selleck Chemicals; cat. no. S5248) for 48 h^{51 (link)}. PKM2 overexpression plasmid and empty vector, PKM2 shRNAs and control were provided by Integrated Biotech Solutions (Shanghai, China). SW579 and TPC-1 cells were (density about 60%) in 6-well plate were transfected with PKM2 overexpression plasmid and vector by applying Lipofectamine 3000 (Invitrogen) in accordance with the reagent instructions. PKM2 shRNAs lentivirus was packaged by WZ Bioscience (Shandong, China). And lentivirus was applied to infect SW579 cells at MOI = 50, and then added with 5 mg/ml Polybrene (Santa Cruz). After 96 h of infection, cells were added with 4 μg/ml Puromycin to screen stable expression cells.