FMRP RNA Binding Assay

RNA binding assay was performed as previously described (6 (link),18 (link)–19 ) using a filter binding assay using Multiscreen Vacuum Manifold and the Multiscreen Separation System (Millipore). A total of 2 pmol of recombinant FMRP were used in each reaction.

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Maurin T., Melko M., Abekhoukh S., Khalfallah O., Davidovic L., Jarjat M., D'Antoni S., Catania M.V., Moine H., Bechara E, & Bardoni B. (2015). The FMRP/GRK4 mRNA interaction uncovers a new mode of binding of the Fragile X mental retardation protein in cerebellum. Nucleic Acids Research, 43(17), 8540-8550.

Publication 2015

Multiscreen Vacuum Manifold Multiscreen Separation System

Assay Fmrp Vacuum

Corresponding Organization : Institut de Pharmacologie Moléculaire et Cellulaire

Other organizations : Institute of Neurological Sciences, National Research Council, Oasi Maria SS, I.R.C.C.S. Oasi Maria SS, Institut de génétique et de biologie moléculaire et cellulaire, Inserm, Collège de France

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Variable analysis

independent variables

Amount of recombinant FMRP used in each reaction (2 pmol)

dependent variables

RNA binding activity

control variables

Filter binding assay using Multiscreen Vacuum Manifold and the Multiscreen Separation System (Millipore)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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