Gene Expression Analysis Protocol

For the expression analysis, 1 µg RNA was used for reverse transcription. The cDNA was synthesized using a FastKing gDNA Dispelling RT SuperMix kit (TIANGEN) in accordance with the manufacturer’s instructions. qRT-PCR was performed using the UltraSYBR Mixture (with ROX; CWBio, Beijing, China) and the CFX96 real-time PCR detection system (Bio-Rad). The expression levels of detected genes were normalized to TUB2 expression. Error bars denote standard deviations of three biological replicates [18 (link)]. The primers used for the expression analysis are listed in Additional file 1.

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Xu X., Xu J., Yuan C., Hu Y., Liu Q., Chen Q., Zhang P., Shi N, & Qin C. (2021). Characterization of genes associated with TGA7 during the floral transition. BMC Plant Biology, 21, 367.

Publication 2021

FastKing gDNA Dispelling RT SuperMix kit UltraSYBR Mixture (with ROX; CFX96 real-time PCR detection system

Biological Cdna Expression genes Primers Reverse transcription

Corresponding Organization :

Other organizations : Hangzhou Normal University

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Variable analysis

independent variables

RNA quantity (1 µg) used for reverse transcription

dependent variables

Expression levels of detected genes

control variables

TUB2 expression used for normalization

controls

No positive or negative controls were explicitly mentioned in the provided information.

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