Fluoro-Jade B (FJB) staining was performed to evaluate neuronal injury in the dorsal hippocampus. Coronal 6 μm paraffin sections were deparaffinized and rehydrated. Slides were immersed in 1% NaOH in 80% alcohol solution for 5 min, then immersed in 70% alcohol and distilled water for 2 min. Slides were blocked in 0.06% potassium permanganate for 20 min before being stained in 0.004% FJB solution in 0.1% acetic acid for 20 min. Slides were rinsed with distilled water, dried on a slide warmer for 10 min, and dipped in xylene and mounted on coverslips with DPX mountant. Images were acquired on a epifluorescent microscope using a 20x objective. 3 ROI’s in 3 sections were obtained per animal. Image analysis was performed by a blinded investigator. Ischemic neurons were characterized by a condensed FJB positive nucleus that did not have chromatin strands or healthy cytoplasm surrounding them.