Spodoptera frugiperda (Sf9) insect cells were obtained
from the American Type Culture
Collection (ATCC, USA). The cells were cultured in HyClone SFX Insect
medium (GE Healthcare, USA) supplemented with the fetal bovine serum
(Gibco). Lucifer yellow (LY, Sigma-Aldrich, USA), 5(6)-carboxy-2′,7′-dichlorofluorescein
(CDCF, Sigma-Aldrich, USA), and N-methyl-quinidine
(NMQ, Solvo Biotechnology, Hungary) were used as substrate probes.
The 44 tested inhibitors were provided by the University of Pittsburgh
Chemical Methodologies and Library Development Center (UPCMLD, USA)
and were described previously.29 (link),30 (link) The remainder of the
chemicals were purchased from Sigma-Aldrich (USA) unless stated otherwise.
from the American Type Culture
Collection (ATCC, USA). The cells were cultured in HyClone SFX Insect
medium (GE Healthcare, USA) supplemented with the fetal bovine serum
(Gibco). Lucifer yellow (LY, Sigma-Aldrich, USA), 5(6)-carboxy-2′,7′-dichlorofluorescein
(CDCF, Sigma-Aldrich, USA), and N-methyl-quinidine
(NMQ, Solvo Biotechnology, Hungary) were used as substrate probes.
The 44 tested inhibitors were provided by the University of Pittsburgh
Chemical Methodologies and Library Development Center (UPCMLD, USA)
and were described previously.29 (link),30 (link) The remainder of the
chemicals were purchased from Sigma-Aldrich (USA) unless stated otherwise.
