Immunoblotting and Immunoprecipitation of Cell Signaling Proteins

Cell lysis, western blotting and immunoprecipitation were carried as described previously (29 (link)). The antibodies used are: anti-RORγt (Cat No: MAB6109, R&D Systems, Inc.), anti-Foxp3 (236A/E7, Cat No:20034, Abcam), anti-Actin (Cat No: A1978, Sigma-Aldrich, Inc.), anti-Stat3 (79D7, Cat No:4904P, Cell Signaling Technology), anti-Stat5 (D2O6Y, Cat No: 94205, Cell Signaling Technology), anti-PRMT1 (Cat No: ab190892, Abcam), anti-PRMT5 (Cat No:ab109451, Abcam), anti-H4R3(me)2a (Cat No: 39705, Active Motif), anti-H4 (Cat No: 61299, Abcam), anti-Foxp3 (236A/E7, Cat No:20034, Abcam), anti-Actin (Cat No: A1978, Sigma-Aldrich, Inc.), anti-Stat3 (79D7, Cat No:4904P, Cell Signaling Technology), anti-Stat5 (D2O6Y, Cat No: 94205, Cell Signaling Technology), anti-PRMT1 (Cat No: ab190892, Abcam), anti-PRMT5 (Cat No:ab109451, Abcam), anti-H4R3(me)2a (Cat No: 39705, Active Motif), anti-H4 (Cat No: 61299, Abcam),

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Sen S., He Z., Ghosh S., Dery K.J., Yang L., Zhang J, & Sun Z. (2018). Critical role of PRMT1 in Th17 differentiation by regulating the reciprocal recruitments of STAT3 and STAT5. Journal of immunology (Baltimore, Md. : 1950), 201(2), 440-450.

Publication 2018

anti-Foxp3 (236A/E7, anti-Actin anti-Stat3 (79D7, anti-Stat5 (D2O6Y, anti-PRMT1 anti-PRMT5 anti-H4R3(me)2a anti-H4

Actin Antibodies Cell Immunoprecipitation Rorγt Stat3 Stat5

Corresponding Organization : City of Hope

Other organizations : University of California, Los Angeles

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Variable analysis

independent variables

Cell lysis
Western blotting
Immunoprecipitation

dependent variables

Protein expression levels of RORγt, Foxp3, Actin, Stat3, Stat5, PRMT1, PRMT5, H4R3(me)2a, H4

control variables

Not explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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