Liver sections from mice livers were routinely fixed in 10% of formalin and embedded in paraffin. Then 5 µm tissue sections were stained with hematoxylin/eosin, oil red and Picrosirius Red, as described previously.24 (link) Whole slide imaging was obtained using the Aperio Digital Pathology Slide Scanner (Leica Biosystems) allowing us to study the entire left lateral lobe. Histopathological analyses were performed by a blinded pathologist starting from three full cross-sectional slides obtained from distal, medial, and proximal regions derived from the left lateral lobe. The blinded trained pathologist utilized the NAFLD Activity Score (NAS) by using the method proposed by Kleiner et al.25 (link)The score of steatoses vary between 0 and 3, the score of ballooning vary between 0 and 2, the score of inflammation vary between 0 and 3, and the score of fibrosis vary between 0 and 4. The NAS is the sum of steatosis, ballooning and inflammation scores. It can vary between 0 and 8. A NAS score less than 3 means no NASH, while a score higher than 4 means NASH. Scores of 3 or 4 means indeterminate.
Protocol detail
Histopathological Assessment of NAFLD in Mice
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Embedded paraffin
Eosin
Fibrosis
Formalin
Inflammation
Liver
Mice
Nafld
Nash
Pathologist
Scanner
Steatosis
Tissue
Corresponding Organization : Imaging Center
Other organizations : Pontificia Universidad Católica de Chile, CeNTech, Universidad Bernardo O'Higgins, University Gastroenterology
Top 5 similar protocols
Variable analysis
independent variables
- Not explicitly mentioned
- Histopathological analyses performed by a blinded pathologist
- NAFLD Activity Score (NAS) including steatosis, ballooning, inflammation, and fibrosis scores
- Liver sections from mice livers were routinely fixed in 10% of formalin and embedded in paraffin
- 5 µm tissue sections were stained with hematoxylin/eosin, oil red and Picrosirius Red
- Whole slide imaging was obtained using the Aperio Digital Pathology Slide Scanner (Leica Biosystems)
- Histopathological analyses were performed by a blinded pathologist starting from three full cross-sectional slides obtained from distal, medial, and proximal regions derived from the left lateral lobe
- Positive control: Not mentioned
- Negative control: Not mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!