Measuring Pain Perception in Mice

Pain perception in male and female was assessed at the age of 13, 15, 17, and 20 months, using the hot plate test as described (Dere et al., 2014 (link)). Briefly, mice were placed on a preheated (55°C) metal plate (Ugo Basile Srl, Comerio, Italy) and the latency (s) to retract by jumping or licking of the hind paws was recorded. Mice were exposed for a maximum of 40 s, used as cut-off time, in case they did not show an aversive response to the heated plate.

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Arinrad S., Depp C., Siems S.B., Sasmita A.O., Eichel M.A., Ronnenberg A., Hammerschmidt K., Lüders K.A., Werner H.B., Ehrenreich H, & Nave K.A. (2023). Isolated catatonia-like executive dysfunction in mice with forebrain-specific loss of myelin integrity. eLife, 12, e70792.

Publication 2023

metal plate

Female Male Metal plate Mice Pain perception

Corresponding Organization :

Other organizations : German Primate Center

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Variable analysis

independent variables

Sex (male and female)

dependent variables

Latency (s) to retract by jumping or licking of the hind paws

control variables

Age (13, 15, 17, and 20 months)
Hot plate temperature (55°C)
Cut-off time (40 s)

Annotations

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