Immunostaining of Stem Cell Markers

The following primary antibodies were used: anti-Krt14 (polyclonal chicken; 1:10,000; BioLegend), anti-GFP (green fluorescent protein; polyclonal rabbit; 1:1000; Abcam, RRID:AB_305564), anti-GFP (polyclonal goat; 1:1000; Abcam, RRID AB_305643), anti-GFP (polyclonal chicken; 1:500; Invitrogen, RRID:AB_2534023), anti-p63 (rabbit monoclonal; 1:1000; Abcam, RRID:AB_10971840), anti-Krt8 (rat monoclonal; 1:250; DSHB, RRID:AB_531826), anti-Entpd2 (rabbit; 1:4000; http://ectonucleotidases-ab.com, mN2-36Li6), anti-Gnat3 (goat polyclonal; 1:500; Novus Biologicals, NBP1-20926), anti-Snap25 (rabbit polyclonal; 1:500; Sigma-Aldrich, S9684), anti-Tas1r2 (rabbit; 1:500; Invitrogen, PA5-99935), anti-Lef1 (rabbit monoclonal; 1:100; Thermo Fisher Scientific, MA5-14966), anti-Sox2 (rabbit monoclonal; 1:200; Abcam ab92494).
The following secondary antibodies were used: anti-rabbit, anti-rat, anti-chicken, anti-goat conjugated to Alexa Fluor 488 (1:500; Jackson ImmunoResearch), to rhodamine Red-X (1:500; Jackson ImmunoResearch), or to Cy5 (1:1000; Jackson ImmunoResearch).

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Vercauteren Drubbel A, & Beck B. (2023). Single-cell transcriptomics uncovers the differentiation of a subset of murine esophageal progenitors into taste buds in vivo. Science Advances, 9(10), eadd9135.

Publication 2023

anti-Krt14 anti-GFP anti-p63 anti-Snap25 anti-Lef1 anti-Sox2 Alexa Fluor 488 rhodamine Red-X

Alexa fluor 488 Antibodies Biologicals Chicken Goat Krt14 Krt8 Lef1 Novus Rabbit Rhodamine Snap25 Sox2

Corresponding Organization : Institut Belge pour la Sécurité Routière

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Variable analysis

independent variables

Anti-Krt14 (polyclonal chicken; 1:10,000; BioLegend)
Anti-GFP (green fluorescent protein; polyclonal rabbit; 1:1000; Abcam, RRID:AB_305564)
Anti-GFP (polyclonal goat; 1:1000; Abcam, RRID AB_305643)
Anti-GFP (polyclonal chicken; 1:500; Invitrogen, RRID:AB_2534023)
Anti-p63 (rabbit monoclonal; 1:1000; Abcam, RRID:AB_10971840)
Anti-Krt8 (rat monoclonal; 1:250; DSHB, RRID:AB_531826)
Anti-Entpd2 (rabbit; 1:4000; http://ectonucleotidases-ab.com, mN2-36Li6)
Anti-Gnat3 (goat polyclonal; 1:500; Novus Biologicals, NBP1-20926)
Anti-Snap25 (rabbit polyclonal; 1:500; Sigma-Aldrich, S9684)
Anti-Tas1r2 (rabbit; 1:500; Invitrogen, PA5-99935)
Anti-Lef1 (rabbit monoclonal; 1:100; Thermo Fisher Scientific, MA5-14966)
Anti-Sox2 (rabbit monoclonal; 1:200; Abcam ab92494)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: None specified
Negative controls: None specified

Annotations

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