The cell viability was assessed employing the ATPlite 1-step Luminescence Assay System (PerkinElmer), as stated earlier17 (link). In brief, cells were seeded in triplicate in 96-well plates at a density of 2 × 103 cells per well, treated for 72 h with different concentrations of VP-16 or in conjunction with KU60019, and then the manufacturer's recommendations for ATPlite cell viability assaying were followed. Plots of the findings from three separate trials were generated.
For clonogenic survival assays, the cells were seeded on 60-mm dishes coated with 0.1% gelatin (Sigma, V900863) and allowed to adhere overnight. After that, the culture medium was refreshed and supplemented with DMSO, KU60019, and VP-16 alone or the combinatorial regimen for the following days. After 7–14 days, cell culture was terminated. For colony counting, colonies were photographed after being stained with coomassie brilliant blue solution.
For clonogenic survival assays, the cells were seeded on 60-mm dishes coated with 0.1% gelatin (Sigma, V900863) and allowed to adhere overnight. After that, the culture medium was refreshed and supplemented with DMSO, KU60019, and VP-16 alone or the combinatorial regimen for the following days. After 7–14 days, cell culture was terminated. For colony counting, colonies were photographed after being stained with coomassie brilliant blue solution.
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