Quantification of Sucrose-Starch Metabolism

Total RNA from the TRs at 50 DAT and 95 DAT were extracted using RNA plant kits (Tiangen, DP441-50T), and then reverse transcribed, and the synthetic cDNA was used for qPCR. In this study, each qPCR result had three biological replications. The primers in this study were shown in Supplementary Table S1. The reference gene was GAPDH, and the relative expression levels related in sucrose-starch metabolism were analyzed using the 2^−ΔΔCT method [45 (link),46 (link)].

Free full text: Click here

Sheng M., Xia H., Ding H., Pan D., He J., Li Z, & Liu J. (2023). Long-Term Soil Drought Limits Starch Accumulation by Altering Sucrose Transport and Starch Synthesis in Sweet Potato Tuberous Root. International Journal of Molecular Sciences, 24(3), 3053.

Publication 2023

RNA plant kits

Biological Cdna Gapdh Gene Metabolism Plant Primers Replications Starch Sucrose

Corresponding Organization : Jiangsu Normal University

Top 5 similar protocols

Variable analysis

independent variables

Days after transplanting (DAT)

dependent variables

Relative expression levels of genes related to sucrose-starch metabolism

control variables

GAPDH (reference gene)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!