After scaled-up synthesis and SPD, TAG species were analyzed by HPLC to further determine which enzyme worked best in producing MLM-type SL TAG species. TAG species determination was performed as described previously with modification 23) (link) . Tricaproin, tricaprylin, tricaprin, trilaurin, triolein and tristearin were used as standards. Their concentrations were at 1 mg/mL after dissolving in dichloromethane. Purified SL was prepared at 5 mg/mL concentration in dichloromethane. Agilent 1260 Infinity Quaternary LC (Agilent Technologies, Inc.) HPLC system equipped with a Sedex Model 85 ELSD (SEDERE SAS Inc., Alfortville, France) was used for TAG species analysis. Separation was achieved with a reverse phase Agilent Zorbax stable bond C18 (250 mm×4.6 mm, 5 µm) column. Column temperature was maintained at 50℃, flow rate at 0.5 mL/ min, injection volume was 5 µL. ELSD drift tube tempera-ture was set at 50℃, nebulizer gas pressure at 3.4 bar and gain at 10. 100％ methanol was used as mobile phase A, and mobile phase B was a mixture of acetone/isopropanol (1:1, v/v) . The following gradient system was employed: hold at 1％ TAG species were determined based on their equivalent carbon number (ECN) compared with the analyzed standards retention times in the chromatograph, and their relative mol％ was calculated based on their peak area.