Evaluating Drug Combinations and siRNA Effects on Cell Growth

50 μl Huh7 (2000 cell/well) and Mahlavu (1000 cell/well) cells were seeded into 96-well plates in 100 μl of medium/well. The next day, cells were treated with the drugs and CI (Cell Index) values were taken every 10 min for 4 h to get the fast drug response and then every 30 min to obtain the long-term drug response. Impedance measurements displayed as CI values reflect cell growth. The SynergyFinder Zero Interaction Potency (ZIP) model is used for the evaluation of the combined effect of PIK-75, TGX-221, and their combinations with Sorafenib (Sorafenib (Nexavar) Bayer Healthcare Pharmaceuticals, Inc., NJ USA). ZIP model defines the effect of combining two compounds by comparing the change in the dose-response curves between individual drugs and their combinations [39 (link)]. For monitoring the effects of siRNA treatment for AOX1 or AGER genes, Mahlavu and Huh7 cells were seeded onto 96-well E-Plate in triplicates. After overnight incubation, siRNA treatments were done as described previously. Cell index (CI) were recorded every 30 min for total of 96 h. Data was normalized using time-zero CI values (when siRNA treatment was performed). For statistical analysis, Two-Way ANOVA was performed using GraphPad Prism 8.