Routine laboratory tests including serum creatinine and albumin-corrected calcium were measured by standard laboratory methods using Roche analysers (Roche Diagnostics Limited, West Sussex RH15 9RY, UK). Estimated glomerular filtration rate (eGFR) was calculated using the MDRD formula [24 (link)]. Serum intact PTH was measured using Roche reagents on the Roche Elecsys 2010 analyser (Roche Diagnostics Limited). Inter- and intra-assay CVs were <5 % at mean concentrations of PTH of 41 and 105 ng/l, respectively.
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