Trophoblast Cell Scratch Assay

The transfected trophoblast cells were cultured in 6-well plates at 1 × 10⁶ cells per well. After complete adherence of cells to the bottom, a 2-mm cell scraper was used to scratch in the middle of each well. The medium and floating cells were washed with PBS. The cell culture was continued in renewed medium at 37°C with 4% CO₂ for 48 h. The width of the scratches at the 0 and 48 h was observed under the microscope (IX51, Olympus) and analyzed using the cellSens standard imaging software (Olympus). The migration rate was cells was measured as follow: rate = (width at 0 h -width at 48 h)/width at 0 h [27 (link)].