Characterization of Ovastacin and ZP2 Antibodies

A rabbit polyclonal antibody that binds a C-terminal peptide of ovastacin^395-408 and monoclonal antibody M2c.2 that binds to the C-terminal region of ZP2 have been characterized previously [32 (link)][45 (link)]. The following antibodies and lectins were obtained commercially: LCA-FITC (Sigma-Aldrich); antibodies to GP73, calregulin and EEA1, Alex Fluor 488 goat anti-rabbit IgG (H+L)(Life Technologies-Invitrogen, Carlsbad, CA); Alexa Fluor 555 donkey anti-rabbit IgG (H+L) (Life Technologies-Invitrogen); DyLight 649 goat anti–rabbit IgG (H+L) (Life Technologies-Invitrogen); and goat anti-rat IgG-HRP (Santa Cruz).

Free full text: Click here

Xiong B., Zhao Y., Beall S., Sadusky A.B, & Dean J. (2017). A Unique Egg Cortical Granule Localization Motif Is Required for Ovastacin Sequestration to Prevent Premature ZP2 Cleavage and Ensure Female Fertility in Mice. PLoS Genetics, 13(1), e1006580.

Publication 2017

LCA-FITC Alexa Fluor 555 donkey anti-rabbit IgG (H+L) goat anti-rat IgG-HRP

Alexa fluor 555 Anti igg Antibodies Antibody Calregulin Donkey Fitc Goat Lectins Monoclonal antibody Peptide c Rabbit

Corresponding Organization : National Institute of Diabetes and Digestive and Kidney Diseases

Top 5 similar protocols

Variable analysis

independent variables

Rabbit polyclonal antibody that binds a C-terminal peptide of ovastacin
Monoclonal antibody M2c.2 that binds to the C-terminal region of ZP2

dependent variables

Not explicitly mentioned

control variables

LCA-FITC (Sigma-Aldrich)
Antibodies to GP73, calregulin and EEA1
Alexa Fluor 488 goat anti-rabbit IgG (H+L) (Life Technologies-Invitrogen, Carlsbad, CA)
Alexa Fluor 555 donkey anti-rabbit IgG (H+L) (Life Technologies-Invitrogen)
DyLight 649 goat anti–rabbit IgG (H+L) (Life Technologies-Invitrogen)
Goat anti-rat IgG-HRP (Santa Cruz)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!