Isolating and Identifying Pseudomonas aeruginosa

Non-duplicate P. aeruginosa isolates were collected from clinics and hospitals from the four district municipalities. Specimens included throat swabs, wound swabs, swabs from abscesses, sputum, urine, blood culture and catheter tips. Demographic characteristics of patients and medical histories were collected from medical records including date of specimen collection, gender and age. All samples were routinely cultured on MacConkey and Blood agar plates. Blood and sputum were also cultured on chocolate agar. Suspected colonies were plated on Cetrimide agar and identified by gram staining, colony characteristics, motility, pyocyanin production and characteristics grape-like odour^{41 (link)}. Strains were identified to the species level with Vitek 2 GN (bioMérieux, Inc. USA) ID cards and confirmed by Microscan NID 2 panels (Beckman Coulter, Inc. USA). Specific primers and probes targeting gyrB were amplified by singleplex rPCR and were also used to confirm identity of the isolates.

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Hosu M.C., Vasaikar S.D., Okuthe G.E, & Apalata T. (2021). Detection of extended spectrum beta-lactamase genes in Pseudomonas aeruginosa isolated from patients in rural Eastern Cape Province, South Africa. Scientific Reports, 11, 7110.

Publication 2021

Vitek 2 GN Microscan NID 2 panels

Abscesses Agar Blood Blood culture Catheter Cetrimide Chocolate Gender Grape Motility Patients Primers Pyocyanin Specimen collection Sputum Strains Throat Urine Wound

Corresponding Organization :

Other organizations : Walter Sisulu University, National Health Laboratory Service

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Identification of P. aeruginosa isolates

control variables

Date of specimen collection
Gender of patients
Age of patients

controls

Positive control: Vitek 2 GN (bioMérieux, Inc. USA) ID cards and Microscan NID 2 panels (Beckman Coulter, Inc. USA) were used to confirm the identity of the P. aeruginosa isolates.
Negative control: Not explicitly mentioned

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