Clinical examinations included questionnaires, medical history, anthropometric measurements, and biochemical analysis. During the examination, the physician recorded the medical history (including previous diseases and drug prescriptions) and drinking frequency and amount. The smoking history was also recorded and distinguished as yes or no.
The anthropometric measurements were performed as previously described, including body weight, standing height, waist circumference, and blood pressure [25 (link), 26 (link)]. Weight and height were measured when the patient was wearing light clothing and no shoes. Waist circumference was measured after the patient exhaled, with the tape measure placed between the lowest rib and the upper edge of the iliac crest. Blood pressure was measured after resting for 5 min. Body mass index (BMI) was calculated as the weight (kg) divided by height (m) squared.
Fasting blood samples were taken from the anterior cubital vein and were used for biochemical analysis. Measurements included liver enzymes, blood lipids, glucose, and uric acid. All of the biochemical values were measured by a Hitachi 7600 clinical analyzer (Hitachi, Tokyo, Japan) using standard methods. Serum 25-hydroxyvitamin D levels were measured with the electro-chemiluminescence immunoassay (ECLIA) platform using the Roche cobas e602 analyzer (Roche Diagnostics GmbH, Germany).
The anthropometric measurements were performed as previously described, including body weight, standing height, waist circumference, and blood pressure [25 (link), 26 (link)]. Weight and height were measured when the patient was wearing light clothing and no shoes. Waist circumference was measured after the patient exhaled, with the tape measure placed between the lowest rib and the upper edge of the iliac crest. Blood pressure was measured after resting for 5 min. Body mass index (BMI) was calculated as the weight (kg) divided by height (m) squared.
Fasting blood samples were taken from the anterior cubital vein and were used for biochemical analysis. Measurements included liver enzymes, blood lipids, glucose, and uric acid. All of the biochemical values were measured by a Hitachi 7600 clinical analyzer (Hitachi, Tokyo, Japan) using standard methods. Serum 25-hydroxyvitamin D levels were measured with the electro-chemiluminescence immunoassay (ECLIA) platform using the Roche cobas e602 analyzer (Roche Diagnostics GmbH, Germany).
Free full text: Click here
