ATF3 Regulation of miR-135b Promoter

70% confluence hESCs were treated with Ad-ATF3-His or Ad-LacZ for 2 days. Crosslink, cell lysis, genomic DNA fragments extraction were performed as described^{32 (link)}, and then immunoprecipitated using ATF3 antibody (ChIP Grade, ab207434, Abcam) and nonspecific IgG as technical control. The specific primers that were used to amplify the hsa-miR-135b promoter DNA fragments containing a putative ATF3 binding sequence were 5′-CAGCTGAAGCCCTCTTTCTG-3′ and 5′-AGGAGGGTCTGGGTAAAGGA-3′.

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Wang Z., Liu Y., Liu J., Kong N., Jiang Y., Jiang R., Zhen X., Zhou J., Li C., Sun H, & Yan G. (2021). ATF3 deficiency impairs the proliferative–secretory phase transition and decidualization in RIF patients. Cell Death & Disease, 12(4), 387.

Publication 2021

ab207434

Antibody Cell Chip Genomic Hescs Hsa mir 135b Lacz Primers

Corresponding Organization :

Other organizations : Nanjing Drum Tower Hospital, Nanjing Medical University, Nanjing University

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Variable analysis

independent variables

Treatment with Ad-ATF3-His or Ad-LacZ

dependent variables

Binding of ATF3 to the hsa-miR-135b promoter DNA fragments

control variables

Confluence of hESCs (70%)
Duration of treatment (2 days)

controls

Positive control: Immunoprecipitation using ATF3 antibody
Negative control: Immunoprecipitation using nonspecific IgG

Annotations

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