In Vivo Assessment of Viral Infection

Animal experiments were performed according to the guidelines of the Institutional Experimental Animal Welfare and Ethics Committee. BALB/c mice aged 42–56 days were purchased from SPF Biotechnology (Beijing, CN) and used for all experimental groups. 100 TCID₅₀ of virus in 10 μL volume was injected intracerebrally into mice under anesthetic conditions (Mishra and Byrareddy, 2020 (link)). Mice in the 4-HT⁺ group and PBS group were administered (intraperitoneal) daily of 0.4 μg 4-HT/g weight of the animal. All mice from each group were sacrificed on day 5 post-inoculation, and their brains were harvested, homogenized, and titered by TCID₅₀ assay or qPCR.

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Zhao Z., Wang B., Wu S., Zhang Z., Chen Y., Zhang J., Wang Y., Zhu D., Li Y., Xu J., Hou L, & Chen W. (2023). Regulated control of virus replication by 4-hydroxytamoxifen-induced splicing. Frontiers in Microbiology, 14, 1112580.

Publication 2023

BALB/c mice

Anesthetic Animal Assay Balb c mice Brains Ethics committee Experimental animal Inoculation Mice Virus

Corresponding Organization :

Other organizations : People's Liberation Army 401 Hospital

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Variable analysis

independent variables

Administration of 4-HT (0.4 μg/g weight of the animal, intraperitoneal, daily)

dependent variables

Viral titer in the brain (measured by TCID50 assay or qPCR)

control variables

Mouse strain (BALB/c)
Mouse age (42-56 days)
Virus dose (100 TCID50 in 10 μL, intracerebrally)
Time point of analysis (day 5 post-inoculation)

controls

PBS group (negative control)

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