Immunoblotting Analysis of Cell Death Markers

Immunoblotting analysis was performed as described previously [21 (link)] using the following primary antibodies: caspase‐3 (1 : 1000; Cell Signaling Technology Inc., Danvers, MA, USA), BCL2‐associated X (BAX; 1 : 1000; Abcam, Cambridge, UK), caspase‐1 (1 : 1000; Abcam), caspase‐11 (1 : 1000; Abcam), GSDMD (1 : 1000; Abcam), NLRP3 (1 : 1000; Cell Signaling Technology Inc.), LC3‐B (1 : 1000; Cell Signaling Technology Inc.), mixed lineage kinase domain‐like protein (MLKL; 1 : 1000; Cell Signaling Technology Inc.), glutathione peroxidase 4 (GPX4; 1 : 1000; Cell Signaling Technology Inc.), proliferating cell nuclear antigen (PCNA; 1 : 1000; Cell Signaling Technology Inc.), and cyclin D1 (1 : 1000; Abcam). GAPDH was used as an internal control.