Phytochemical Analysis of Rosemary Extract by GC-MS

Shimadzu QP-2010 GC-MS system equipped with a capillary column (inner diameter 0.25 mm and length 30 m) was used to analyse the constituents of the EtOH extract of R. officinalis [15 (link)]. The temperature of the GC oven was kept at 100 °C for two minutes and was further programmed to 280 °C at the rate of 10 °C/min and then kept at 280°C for 13 min. The split ratio was 1: 25 and the injection volume was 2 μL. The injection port and detector port temperatures were 200°C and 240°C, respectively. The GC-MS electron ionization mode was 150 eV. The mass scanned was between m/z 20–500 amu (70 ms accumulation time). The peaks of the gas chromatogram were subjected to mass spectral analysis. The active constituents were identified based on the retention indices and by the comparison of mass spectra with the National Institute of Standards and Technology library of mass spectral data.