As described above, determination of the number of subjects to be enrolled in the present study was based on a combination of data from other published studies in our laboratory and a pilot study specific to the subject population and exercise protocol (McFarlin et al., 2006 (link), 2013 (link); Strohacker et al., 2012 (link); Carpenter et al., 2013 (link)). Over a period of 2 years, we enrolled subjects until we reached our target of over 100 total subjects completing the entire study protocol. College-aged men and women who were of average physical activity status and who were not actively exercising were recruited. Subjects were screened for inclusion/exclusion criteria using a written medical history form, a whole body DXA scan for body composition (GE Lunar Prodigy; USA), and a graded exercise test on a treadmill. Detailed subject characteristics are presented in Table 1.
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