Cell Invasion Assay with Spheroid Disintegration

Cell invasion assay was carried out using the BD Falcon^™ BioCoat tumour invasion systems (Corning, Arizona, USA) with fluoroBlock^™ 96 well insert plate according to manufacturer’s guidelines. This was also compared with migration of cells through uncoated BD Falcon™ FluoroBlok^™ 96 well insert plates (see Balahmar et al. 2018 (link)) for details). In this study, spheroid cells were used that have a total diameter above 8.0 μm. Therefore, the spheroids were disintegrated by using trypsin into single cells before performing the invasion assay. The number of cells invaded from the tumour invasion plate together with percentage invasion (see below for equation) was analysed using Image J software. $$\text{Invasion}\%=\frac{\text{Number of cells invaded}}{\text{Number of cells migrated}}\times 100.$$